![]() For example, the fixation and permeabilization processes during intracellular staining can alter cell size and granularity, resulting in modified forward and side scatter profiles (discussed below). Things to determine are the relative expression levels of cell specific markers, the approximate size of the cells, and whether their size can be affected by experimental conditions. Therefore, before you begin your analysis, it is important to first find out as much as possible about the cells you are analyzing. Your gating strategy is informed by what you know about your cells of interest. Learn as much as possible about your cells of interest ![]() So what gating methods do you need to know to confidently analyze your stained samples? This blog post will take you through the various gating strategies for effective flow cytometry analysis. This process of gating can appear quite random to a flow cytometry novice but it is in fact the most important part of flow cytometry analysis. Flow cytometry analysis typically begins with creating gates to distinguish cells of interest.
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